Cloning

In this category, Gene and PCR Cloning, we have the following subcategories (product groups):

  • Ligases: Regular and fast ligases you can use to set up ligation reactions for cloning and adding adaptors to your DNA. 
  • TA-cloning Kits: For cloning PCR products with 3'-protruding A's (by Taq DNA polymerase), competent cells included. 
  • Blunt-end Cloning Kits: For cloning PCR products with blunt ends (by proof-reading DNA polymerase). Competent cells included.
  • Universal Cloning Kits: Cloning PCR products or other DNA, without needing restriction endonuclease sites. Ligation-free. Competent cells included
  • Competent Cells: A large selection of commonly used competent cells. 

Cloning There are 33 products.

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Showing 1 - 12 of 33 items
  • CAD$75.00

    T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme.Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA,RNA and DNA/RNA, are possible via the T4 DNA ligase. Use to clone gene fragments or PCR products.

    CAD$75.00
  • CAD$99.00

    Trans1-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^9 cfu/μg DNA (tested by pUC19 plasmid DNA). • High efficiency: >10^9 cfu/μg. • Fast-growth. • Resistance to T1 and T5 phage. • Blue/white selection.

    CAD$99.00
  • CAD$296.00

    pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning Pfu-amplified PCR products. •5 min fast ligation •Ampicillin resistance marker •Blue/white selection •T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. •Includes Competent Cell, high efficiency (>10^9 cfu/μg...

    CAD$296.00
  • CAD$144.00

    Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR), featuring: High efficiency: >10^8 cfu/μg ( pUC19 DNA)./StrR./ Blue/white selection/Toxic gene...

    CAD$144.00
  • CAD$288.00

    Size: 20 reactions (10x100ul) TransDB3.1 Chemically Competent Cells are for chemical transformation of DNA. This cell contains the gyrA462 gene harboring resistance to the toxic effects from the ccdB gene. TransDB3.1 Chemically Competent Cell can be used for transformation and propagation of plasmid containing the ccdB gene. It permits a transformation...

    CAD$288.00
  • CAD$177.00

    Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR), featuring: /High transformation efficiency: >10^8 cfu/μg ( pUC19 DNA)/TetR /Blue/white selection.

    CAD$177.00
  • CAD$123.00

    DMT Chemical Competent cells have high transformation efficiency: >10ˆ8 cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols.

    CAD$123.00
  • CAD$377.00

    pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required. •5 minutes fast ligation •High cloning efficiency. Positive clones up to 100% •No blue/white selection needed

    CAD$377.00
  • CAD$124.00

    Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). High efficiency: >10^8 cfu/μg ( pUC19 DNA)./Reduced recombination of cloned DNA./Blue/white selection.

    CAD$124.00
  • CAD$296.00

    pEASY ®-T1 Cloning Kit is designed for cloning Taq-amplified PCR products. •5 minutes fast ligation of PCR products. •Choice of Kanamycin or Ampicillin resistance genes. •Blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing. •Includes Trans1-T1 Phage Resistant Chemical Competent Cells, high efficiency (>10^9 cfu/μg...

    CAD$296.00
  • CAD$215.00

    This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. •Fast: 15 min •Broad: no restriction enzyme digestions. Can be cloned into any sites •Up to 95% cloning efficiency •Seamless: no extra sequences...

    CAD$215.00
  • CAD$234.00

    Trans2-Blue Chemically Competent Cells are for chemical transformation of DNA. It permits a transformation efficiency of over 10^9 cfu/μg DNA (tested by pUC19 plasmid DNA). The cells areresistant to tetracycline (TetR) and chloramphenicol (CamR), featuring: /High efficiency (>1×10^9cfu/μg DNA)/For larger plasmids and recombinant products/library...

    CAD$234.00
Showing 1 - 12 of 33 items