Cloning

In this category, Gene and PCR Cloning, we have the following subcategories (product groups):

  • Ligases: Regular and fast ligases you can use to set up ligation reactions for cloning and adding adaptors to your DNA. 
  • TA-cloning Kits: For cloning PCR products with 3'-protruding A's (by Taq DNA polymerase), competent cells included. 
  • Blunt-end Clon...

In this category, Gene and PCR Cloning, we have the following subcategories (product groups):

  • Ligases: Regular and fast ligases you can use to set up ligation reactions for cloning and adding adaptors to your DNA. 
  • TA-cloning Kits: For cloning PCR products with 3'-protruding A's (by Taq DNA polymerase), competent cells included. 
  • Blunt-end Cloning Kits: For cloning PCR products with blunt ends (by proof-reading DNA polymerase). Competent cells included.
  • Universal Cloning Kits: Cloning PCR products or other DNA, without needing restriction endonuclease sites. Ligation-free. Competent cells included
  • Competent Cells: A large selection of commonly used competent cells. 
More

Cloning There are 18 products.

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Showing 1 - 12 of 18 items
  • CAD$75.00

    GB-Clone™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme.Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA,RNA and DNA/RNA, are possible via the GB-Clone™ T4 DNA ligase. Use to clone gene fragments or...

    CAD$75.00
  • CAD$296.00

    pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning Pfu-amplified PCR products. •5 min fast ligation •Ampicillin resistance marker •Blue/white selection •T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. •Includes Competent Cell, high efficiency (>10^9 cfu/μg...

    CAD$296.00
  • CAD$377.00

    pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required. •5 minutes fast ligation •High cloning efficiency. Positive clones up to 100% •No blue/white selection needed

    CAD$377.00
  • CAD$296.00

    pEASY ®-T1 Cloning Kit is designed for cloning Taq-amplified PCR products. •5 minutes fast ligation of PCR products. •Choice of Kanamycin or Ampicillin resistance genes. •Blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing.

    CAD$296.00
  • CAD$215.00

    This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. •Fast: 15 min •Broad: no restriction enzyme digestions. Can be cloned into any sites •Up to 95% cloning efficiency •Seamless: no extra sequences...

    CAD$215.00
  • CAD$296.00

    pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning Pfu-amplified PCR products. •5 min. fast ligation •choice of resistance genes markers •Blue/white selection •SR primer, M13 forward primer for sequencing. •Trans1-T1 Phage Resistant Competent Cell, high efficiency (>10^9...

    CAD$296.00
  • CAD$296.00

    pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released with a single enzyme digestion. •5 minutes fast ligation •Ampicillin resistance gene marker •Blue/white selection •T7, SP6 promoters, M13 forward and M13 reverse primers for...

    CAD$296.00
  • CAD$296.00

    pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing. •Trans1-T1 Phage Resistant Chemical Competent Cell, high efficiency (>10^9 cfu/μg...

    CAD$296.00
  • CAD$90.00

    GB-Clone™ FAST T4 DNA Ligase is designed for the efficient and rapid ligation of cohesive-ended DNA insertsinto plasmid vectors in just 5-10 minutes (blunt-ended inserts in as little as 15-30 minutes).FAST T4 DNA Ligase catalyzes the joining of two strands of DNA between the5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either...

    CAD$90.00
  • CAD$296.00

    pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 min. fast ligation •choice of resistance genes •Blue/white selectio •SR primer and M13 forward primer for sequencing

    CAD$296.00
  • CAD$377.00

    pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed.

    CAD$377.00
  • CAD$187.00

    The method works  first by having primers anneal to the DNA template.  Mutant strands are then synthesized with 2×TransStart® FastPfu PCR SuperMix with high fidelity. In the next step, non-mutated parental plasmid (methylated plasmid) is digested in vitro with DMT enzyme and in vivo further digested within DMT Chemically Competent Cell. 

    CAD$187.00
Showing 1 - 12 of 18 items