pEASY®-T3 Cloning Kit

pEASY®-T3 Cloning Kit

CT301
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$296.00 CAD
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$296.00 CAD
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Description

pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.

•5 minutes fast ligation of Taq-amplified PCR products.

•Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter and SP6 promoter for in vitro transcription.

•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

At -20°C for nine months

Kit Content

Component

CT301-01

CT301-02

(20 rxns)

(60 rxns)

pEASY ®-T3 Cloning Vector (10 ng/μl)

20µl

3×20µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

SR Primer (10 μM)

Note: this kit does not include competent cells. 

References:

Literature Journal IF Author Date Link
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching. Nature Biotechnology 39.08 2013 Sep The original link
Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice Genome Biology 10.465 Yu-Chan Zhang, et al. 2014 Dec The original link
Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years journal of hazardous materials 6.065 Shuang Peng,et al. 2017Qct The original link
Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli Scientific Reports 5.578 Jie Gao, et al. 2016 Jan The original link
Identification of ethylene-responsive genes in ethrel-treated shoot apices of cucumber by suppression subtractive hybridization Plant Molecular Biology Reporter 5.319 Jin X, et al. Department of Horticulture, Northeas 2011 Dec The original link