I have been very impressed with this column-based kit for RNA isolation. I have been using the kit to isolate RNA from cultured pre-adipocytes and adipocytes that had been previously lysed and stored at -80oC in Lysis reagent (eg. TrAZol). The kit is very easy to use, the protocol is quick, and it does not require the use of other more toxic compounds, such as choloroform, as is required by other kits. I was very happy with my RNA yields, which were comparable to what I've obtained using much more expensive RNA isolation kits from competitors, and more importantly the RNA purity was superior to that obtained in the other kits, as assessed by A260/A280 and A230.