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Description |
pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter for in vitro transcription.
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Storage |
Competent Cell at -70°C for six months; others at -20°C for 9 months |
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Kit Content |
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Component |
CB101-01 |
CB101-02 |
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(20 rxns) |
(60 rxns) |
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pEASY ®- Blunt Cloning Vector (10 ng/μl) |
20µl |
3×20 µl |
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Control Template (5 ng/μl) |
5µl |
5µl |
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Control Primers (10 μM) |
5µl |
5µl |
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M13 Forward Primer (10 μM) |
50µl |
150µl |
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M13 Reverse Primer (10 μM) |
50µl |
150µl |
Please note: this kit does not include competent cells.
References
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew | Nature Biotechnology | 39.08 | Yanpeng Wang, et al. | 2014 Jul | The original link |
| D14?CSCFD3-dependent degradation of D53 regulates strigolactone signaling | Nature | 38.597 | Feng Zhou,et al. | 2013 Dec | The original link |
| ??Perfect?? designer chromosome V and behavior of a ring derivative | Science | 34.661 | Xie Z, et al. Key Laboratory of Systems Bioenginee | 2017 Mar | The original link |
| Targeted genome modification of crop plants using a CRISPR-Cas system | Nature Biotechnology | 32.44 | Qiwei Shan,et al. | 2013 Aug | The original link |
| Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds | Cell Research | 12.413 | Junqing Ye, et al. | 2015 Dec | The original link |