pEASY®-T1 Cloning Kit

pEASY®-T1 Cloning Kit

CT101
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$296.00 CAD
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$296.00 CAD
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Description

pEASY ®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

At -20°C for 9 months

Kit Content

Component

CT101-01

CT101-02

(20 rxns)

(60 rxns)

pEASY ®-T1 Cloning Vector (10 ng/μl)

20µl

3×20 µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

l

Please note: Comp cells are available separately, but not included in the kit. 

References:

Literature Journal IF Author Date Link
A maize wall-associated kinase confers quantitative resistance to head smut Nature Genetics 29.648 Weiliang Zuo et al. 2014 Dec The original link
An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx mori Molecular Biology and Evolution 14.308 Wei Sun, et al. 2014 Oct The original link
pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements Life 9.322 Xiaoyu Xiang, et al. 2015 Feb The original link
Undifferentiated embryonic cell transcription factor-1 (UTF1) inhibits the growth of cervical cancer cells by transactivating p27 Kip1 Carcinogenesis 5.635 Wu X L, Zheng P S. Department of Reproductive Medi 2013 Jul The original link
Differential regulation of taurine biosynthesis in rainbow trout and Japanese flounder Sci Rep. 5.578 Xuan Wang, et al. 2016 Feb The original link