|
Description |
T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 150 μg of RNA can be produced from a 20 μl reaction. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays. |
|
Storage |
at -20°C for one year |
|
Kit Content |
|
Component |
JT101-01 (25 rxns) |
|
T7 Transcription Enzyme Mix |
50 µl |
|
5×T7 Transcription Reaction Buffer |
100 µl |
|
10 mM NTP Mix |
200 µl |
|
DNase I (1 unit/µl) |
25 µl |
|
500 mM EDTA (pH 8.0) |
25 µl |
|
RNase-free Water |
500 µl |
|
Control Transcription Template (0.5 µg/µl) |
10 µl |