This kit can yield 150 - 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications
such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.
Kit Composition
| Components | TR101-01 50 rxn | TR101-02 100 rxn |
| T7 RNA Polymerase Mix | 100 μl | 200 μl |
| 10 × Reaction Buffer | 100 μl | 200 μl |
| ATP Solution | 100 μl | 200 μl |
| UTP Solution | 100 μl | 200 μl |
| GTP Solution | 100 μl | 200 μl |
| CTP Solution | 100 μl | 200 μl |
| DNase I | 50 μl | 100 μl |
| Control Template (0.5 μg/μl) | 10 μl | 20 μl |
| RNase-free H2O | 1 ml | 2 x 1 ml |
Storage: Store all the components at -20℃.