pEASY®-Uni Seamless Cloning and Assembly Kit

pEASY®-Uni Seamless Cloning and Assembly Kit

CU101
Regular price
$215.00 CAD
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$215.00 CAD
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Description

This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.

•Fast: 15 minutes.

•Broad: no restriction enzyme digestions. Can be cloned into any sites.

•High efficiency: up to 95% cloning efficiency.

•Seamless: no extra sequences introduced; up to 5 fragments assembly.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CU101-01

CU101-02

CU101-03

2×Assembly Mix

50 μl

100 μl

3×100 μl

Linearized pUC19 Control Vector (10 ng/μl)

3 μl

6 μl

18 μl

Control Insert (1 kb,20 ng/μl)

3 μl

6 μl

18 μl

Please note: this kit does not include competent cells. 

References:

Literature Journal IF Author Date Link
Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway METABOLIC ENGINEERING 8.142 Wang Y, et al. State Key Laboratory of Microbial M 2017 Jan The original link
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system new phytologist 7.21 Borong Lin, et al. 2015 Oct The original link
Phosphorylation-mediated Regulatory Networks in Mycelia of Pyricularia oryzae Revealed by Phosphoproteomic Analyses MOLECULAR & CELLULAR PROTEOMICS 6.5399 Ruijin Wang,et al. 2017Jul The original link
The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles. Plant journal 5.4679 Li S, et al. Biotechnology Research Institute, Chi 2015 Oct The original link
Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation. Scientific Reports 5.2278 Hu H, et al. State Key Laboratory of Microbial Met 2015 Dec The original link