The FITC-conjugated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence.PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-FITC and PI staining allows the differentiation among different stage of apoptotic cells and necrosis cells.
|
Storage |
at 4°C in dark for one year. |
|
Kit Content |
|
Component |
FA101-01 (25 rxns) |
FA101-02 (50 rxns) |
|
Annexin V-FITC |
125 μl |
250 μl |
|
Propidium Iodide (PI) |
125 μl |
250 μl |
|
1× Annexin V Binding Buffer |
12.5 ml |
2×12.5 ml |
Literature citing this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells | Oncotarget | 6.359 | Chengle Zhuang, et al. | 2015 Oct | The original link |
| Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancer effect with glycolysis inhibition | Cancer Letters | 5.621 | Rui Ju, et al. | 2015 Oct | The original link |
| Acetylation of p53 at lysine 120 upregulates Apaf-1 and sensitizes mitochondrial apoptotic pathway | JOURNAL OF BIOLOGICAL CHEMISTRY | 4.573 | Tao Yun, et al. | 2016 Feb | The original link |
| Intracellular calcium promotes radioresistance of non?Csmall cell lung cancer A549 cells through activating Akt signaling | tumor biology | 3.65 | Yiling Wang,et al. | 2017Mar | The original link |
| FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells | applied microbiology and biotechnology | 3.42 | Shuying Li,et al. | 2017Apr | The original link |