Collection: PCR for Difficult Targets

Usually after a routine PCR attempt, researchers would find that their PCR has failed to amplify the intended target into the correct product. The reasons for this failure can be a few, top on the list, is the complexity of PCR target. Specifically, when the target sequence, or the primer sequence, contains high GC content >60%, or if the target sequence contains a secondary structure, the targets are difficult to denature, leading to failed PCR. The solution to this problem is to use the PCR for Difficult Targets. This type of PCR is featured by using polymerases that are better able to handle the high complexity targets, or additives such as the PCR Enhancing Reagent. 

7 products
  • 2x GB-AMP™ PaCeR™ HP™ Master Mix
    PaCeR™ HF™ DNA polymerase
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    from $86.00 USD
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    from $86.00 USD
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  • 2X Phanta Max Master Mix
    PaCeR™ HF™ DNA polymerase
    Regular price
    from $86.00 USD
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    from $86.00 USD
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  • GB-AMP™ PaCeR™ HP™ DNA polymerase
    PaCeR™ HF™ DNA polymerase
    Regular price
    from $97.00 USD
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    from $97.00 USD
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  • 2X Achiever™ Taq Master Mix (with loading dye)
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    from $117.00 USD
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    from $117.00 USD
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    $702.00 USD
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  • Achiever(TM) DNA Polymerase
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    from $32.00 USD
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    from $32.00 USD
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  • TransStart® FastPfu Fly DNA Polymerase
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    from $211.00 USD
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    from $211.00 USD
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  • PCR Enhancing Reagent
    PCR Enhancing Reagent
    Regular price
    from $15.00 USD
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    from $15.00 USD
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