Collection: In Vitro Transcription

In vitro transcription involves the synthesis of RNA molecules from a DNA template using the enzyme RNA polymerase. Our kits provide a DNA template, RNA polymerase enzyme, nucleotides, and buffer solutions optimized for transcription reactions. Learn more about exciting uses of our in vitro transcription kits:

1. RNA Synthesis for Molecular Biology Studies:

Researchers can synthesize RNA with specific sequences for functional assays, RNA structure studies, or as molecular tools for manipulating gene expression.

2. Generation of RNA Probes:

In situ hybridization and other nucleic acid detection techniques often require labeled RNA probes. Our in vitro transcription kits are capable of generating these probes by incorporating labeled nucleotides (i.e., radioactive, fluorescent, or biotinylated) during transcription.

3. Gene Expression Studies:

Our kits can be used to generate RNA transcripts, transcribe specific gene sequences, and analyze the resulting RNA molecules for studying gene expression to understand gene regulation and function.

4. RNA Structure and Function Studies:

In vitro transcribed RNA molecules are valuable tools for studying RNA structure and function. They can be used in experiments such as RNA folding studies, ribonucleoprotein complex formation, and investigations into RNA-protein interactions.

5. Synthesis of Modified RNA:

In vitro transcription can be adapted to incorporate modified nucleotides, allowing the synthesis of RNA with specific chemical modifications. This is useful for studying the effects of modifications on RNA structure and function.

6. RNA Aptamer Selection:

Aptamers are short RNA sequences that can bind to specific targets with high affinity. In vitro transcription kits are used in the generation of RNA libraries for the selection and amplification of aptamers through techniques like Systematic Evolution of Ligands by Exponential Enrichment (SELEX).

7. RNA Vaccines and Therapeutics:

RNA vaccines, such as messenger RNA (mRNA) vaccines, use in vitro transcribed RNA to express antigens in cells, triggering an immune response.

8. RNA Delivery Systems:

In vitro transcribed RNA can be used in the development of RNA-based delivery systems. Researchers can engineer RNA molecules with specific properties for therapeutic applications, including RNA interference (RNAi) and gene therapy.

9. RNA-Based Functional Genomics

In vitro transcribed RNA molecules can be used in functional genomics studies to perturb gene expression in a controlled manner. Techniques like RNA interference (RNAi) use synthetic RNA to selectively silence target genes.

Explore our kits to find the best fit for your research:

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  • 100mM rNTP Set
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  • T7 RNAi Transcription Kit
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  • miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)
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  • T7 High Yield RNA Transcription Kit
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  • T7 High Efficiency Transcription Kit
    T7 High Efficiency Transcription Kit
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