DNA ligases are enzymes that catalyze the formation of phosphodiester bonds between adjacent DNA fragments. Specifically, DNA ligases seal nicks or gaps in the DNA backbone, resulting in the formation of a continuous, intact DNA strand. Their mechanism of action involves the use of energy derived from ATP or NAD+ to join the 3’-hydroxyl end of one DNA strand to the 5’-phosphate end of another to form a phosphodiester bond. These enzymes are essential in various cellular processes, including DNA replication, repair, and recombination, in addition to cloning, gene synthesis and assembly, site-directed mutagenesis, construction of DNA libraries, creation of chimeric DNA molecules, and NGS library preparation.
We carry a variety of DNA ligases that can be tuned to your experimental needs
