Spectrophotometry is a technique used to measure the intensity of light absorbed by a sample at different wavelengths. Traditional spectrophotometers operate in the UV-visible range (typically from 190 to 1100 nm), but nano spectrophotometers are capable of measuring at smaller scales. Nano spectrophotometers are specifically designed to measure the absorbance, transmittance, and concentration of samples with high sensitivity, particularly in the nanometer scale.
Nano Spectrophotometers typically consist of the following components:
Light Source
They employ a light source that emits a broad spectrum of wavelengths, often including UV, visible, and near-infrared light.
Monochromator or Filters
A monochromator or a set of filters is used to select specific wavelengths of light for measurement.
Sample Holder
Nano spectrophotometers may have specialized sample holders designed to accommodate very small sample volumes, often in the microliter or even nanoliter range.
Detector
They use highly sensitive detectors capable of measuring very low levels of light intensity.
Software Interface
Most nano spectrophotometers come with software interfaces for data collection, analysis, and interpretation.
Our nano spectrophotometers are commonly used in:
Nucleic Acid and Protein Quantification: Nano spectrophotometers are commonly used for quantifying nucleic acids (DNA, RNA) and proteins. They can accurately determine the concentration of nucleic acids or proteins in a sample by measuring their absorbance at specific wavelengths. This is crucial for various molecular biology techniques such as PCR, qPCR, and protein assays.
Quality Control in DNA Sequencing and Synthesis: In DNA sequencing and synthesis applications, accurate quantification of DNA is essential for ensuring the success and reliability of experiments. Nano spectrophotometers provide precise measurements, helping researchers optimize reaction conditions and troubleshoot issues.
Nanoparticle Characterization: Nano spectrophotometers are also used for characterizing nanoparticles and nanomaterials. They can measure the optical properties of nanoparticles, including absorbance and transmittance spectra, which are crucial for understanding their behavior and applications in fields such as nanomedicine, catalysis, and nanoelectronics.
Cell Culture and Microbiology: In cell culture and microbiology research, nano spectrophotometers can be used to quantify cell density and biomass concentration. By measuring absorbance at specific wavelengths, researchers can estimate the number of cells or the concentration of microbial biomass in a culture.
Material Science: Nano spectrophotometers find applications in material science for analyzing the optical properties of nanomaterials, thin films, and coatings. Researchers use them to characterize materials based on their absorbance and transmittance spectra, providing insights into their composition, structure, and performance.
Nano Spectrophotometer Brochure
