A polymerase chain reaction (PCR) is a crucial technique used to rapidly produce copies of a specific part of a DNA in masses-usually trillions of copies. Reagents in this context are the biochemical components, such as polymerases, dNTP, magnesium, minerals and buffers that PCR requires to duplicate a genetic material successfully.
Components of PCR Reaction
The primary ingredients necessary for a complete PCR reaction include:
DNA template: this is a sample DNA containing the target sequence
DNA polymerase: a unique enzyme that synthesizes new DNA strands to complement the target sequence
Primers: short pieces of single-stranded DNA which complement the target sequence
Nucleotides: building blocks for new DNA strands
PCR Amplification Reaction
Gene Bio Systems emphasizes that the success of the PCR technique is part of many molecular biological applications. Our range of products supports various processes, including PCR amplification for difficult to amplification templates, for applications in which the fidelity of amplification is important, for applications in which the DNA template may not have been fully purified. We have the PCR reagents for most of the common molecular applications.
Like other scientific processes, it is worth noting that PCR reactions exhibit a lot of complexity. The success of various PCR amplification reactions depends on the quality and performance of appropriate PCR enzymes, master mixes and ancillary reagents. The products should be fully qualified for the stated applications. Contaminants/impurifies in the reagents, or anything less than robust and rational design in the reagents set back the entire PCR process; thus, getting products from a reliable source is crucial. Gene Bio Systems delivers exactly that. You can buy our products online and reach us for more support.