Hyperactive pG-MNase CUT&RUN Assay Kit for PCR/qPCR is developed specially for studying protein-DNA interactions. Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is a new method for studying protein-DNA interactions. By fusing Protein G with transposase, under the guidance of the antibody, it allows accurate targeting of the target protein and cutting of the DNA sequence near the target site. With an optimized reaction system and workflow, this kit offers multiple advantages over conventional ChIP-qPCR, including high success rate, excellent antibody compatibility, short turnaround time, and ease of use, making It is especially suitable for such fields as early embryonic development, stem cells, cancer, and epigenetics. All reagents in this kit have undergone strict quality control and function testing to ensure the optimal stability and repeatability of experimental results.
06-1/About qPCR Reagents
This product is intended for studying protein-DNA interactions in mammalian cells with
5,000 - 500,000 input cells. Yeast, plant tissues and cells should be treated before proceeding
with the assay using this kit.