HiScript® II Q Select RT SuperMix for qPCR

HiScript® II Q Select RT SuperMix for qPCR

R232
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$196.00 USD
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$196.00 USD
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Size: 200 rxn (10 μl/rxn)

• Based on high effective HiScript II Reverse Transcriptase.

• Flexible options of RT primers for different applications.

• Ready-to-use SuperMix.

The HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR is designed for 2-step RT-qPCR. The 5× HiScript II Select qRT SuperMix contains Buffer, dNTPs, HiScript II Reverse Transcriptase, and RNase inhibitor. The Oligo (dT)23VN has a better affinity to Ploy ARNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.

The HiScript II Q Select RT SuperMix for qPCR has been specially optimized for qPCR. The cDNA products are compatible for SYBR- or probe-basded qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).

 

R211

R212

R222

R223

R232

R233

Application

 

 

 

 

 

 

RT-qPCR

 

 

universal

 

 

 

 

Features

 

 

 

 

 

 

Ready-to use

 

 

Long cDNA synthesis

 

 

gDNA wiper

 

 

 

Primers

 

 

 

 

 

 

Oligo (dT)23VN/N6 Mix

 

 

 

 

self-selection

 

 

Table 1. Guidelines for choice of HiScript II two-step RT-PCR/qPCR series.