Gel-PCR 2in1 Kit- HiPure  DNA Kit

Gel-PCR 2in1 Kit- HiPure DNA Kit

D211101
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Gel-PCR 2in1 Kit-HiPure Gel DNA Mini Kit

D2111

CAT NO PRODUCT NAME SIZE
D211102 HiPure Gel DNA Mini Kit 100 preps
D211103 HiPure Gel DNA Mini Kit 250 preps


Introduction

Gel-PCR 2in1 Kit- HiPure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details


Specifications

Features Specifications
Main Functions Recover DNA fragments between 60bp-10kbp from agarose gel
Applications PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Agarose gel, PCR products, enzyme products
Sample amount
Agarose gel: ≤500mg
Recovery ≥80%
Elution volume ≥15μl
Time per run ≤20 minutes(1-24 samples)
Liquid carrying volume per column 800µl
Binding yield of column 35µg


Principle

The Gel-PCR 2in1 Kit-HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Now, keep this one kit in your lab and use it for your gel extraction purification or your PCR or other enzymatic reaction products, it is your choice which application you need to use the kit for!

Comparing to four other leading brands (M is GeneBio-Magen's product)-see below. 

Advantages

  • High recovery efficiency - ≥80% DNA recovery
  • General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
  • Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents D211101 D211103
Number of Preps
50 Preps 250 Preps
Buffer GDP 50 ml 250 ml
Buffer DW2 20 ml 2 x 50 ml
Elution Buffer
6 ml
30 ml
HiPure DNA Mini Columns II
50
250
2 ml Collection Tubes 50 250


Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.