Purification
The usage of GB-Magic DNA Beads is the same as the AMPure® XP Beads (Beckman, Cat.No. #A63881), which is widely used in PCR purification, enabling reaction clean-up, after ligation and PCR steps in general
GB-Magic effectively removes salts, primers, primer-dimers (<100 bp) and dNTPs. The protocol can be performed manually, or adapted to liquid handling workstations (e.g. Agilent, Beckman, Caliper, Eppendorf, Hamilton, PerkinElmer and Tecan) using individual tube, 96- or 384-well formats.
Size Selection
In addition to workflows in NGS for cleanup (purification), GB-Magic DNA Beads are also used in NGS library preparation for Size Selection. The yield and size distribution of the libraries prepared with GB-Magic DNA Beads are equivalent with those with the SPRIselect bead-based reagents such as SPRIselect and other reagents. The cost-effective GB-Magic DNA Beads makes it easy to replace the SPRIselect Bead-based reagents such as SPRIselect in your current protocols.
Applications
GB-Magic is perfectly suited to the following applications:
• NGS library preparation-follow size selection and purification protocols below
• Sanger sequencing-follow purification protocol below
• PCR-follow purification protocol below
• Recovery from enzymatic reactions-follow purification protocol below
