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EasyPure® Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.
- Fast extraction, high yield
- up to 15 μg
- RNase A and Proteinase K included
- High integrity of gDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.
- High purity enabled by spin column which can efficiently and specifically bind to DNA and removes protein, salts, lipids or other contaminants.
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
| Component | EE101-01 (50 rxns) |
| Lysis Buffer 2 (LB2) | 6 ml |
| Binding Buffer 2 (BB2) | 28 ml |
| Clean Buffer 2 (CB2) | 15 ml |
| Wash Buffer 2 (WB2) | 12 ml |
| Elution Buffer (EB) | 25 ml |
| RNase A (20 mg/ml) | 1 ml |
| Proteinase K (20 mg/ml) | 1 ml |
| Genomic Spin Column with Collection Tubes | 50 each |
Sample requirement
| Material | Amount |
| Mammalian cells | 1-5x10^6 cells |
| Mammalian tissues | ≤25 mg |
| Mouse tail | 0.5 cm sections |
| E. coli cells | ≤2x10^9 cells |
| Yeast cells | ≤1x10^7 cells |
Some samples extracted using EE101
|
Mouse Samples |
Concentration (ng/μl) |
A260/A280 | A260/A230 |
|
Uterus(01) Ovary(02) Kidney(03) liver (04) lungs(05) Heart (06) Brain (07) Spleen (08) Mesenteric lymph nodes (09) Inguinal lymph nodes (10) Drug delivery site (11) Skin(12) Sciatic nerve (13) Bone marrow (14) Spinal cord (15) Blood (16) |
173.92 120.39 130.77 141.34 93.51 111.63 90.23 464.25 637.62 67.87 165.77 238.27 20.73 109.58 79.54 60.40 |
1.92 1.92 1.93 1.93 1.93 1.92 1.95 1.95 1.95 1.94 1.92 1.93 1.93 1.91 1.94 2.13 |
2.3 2.26 2.1 2.12 2.19 2.05 1.94 2.42 2.35 1.75 2.14 2.27 1.82 2.09 2.13 1.22 |
| Cynomolgus monkey samples |
Concentration (ng/μl) |
A260/A280 | A260/A230 |
|
Testicles (01) Epididymis (02) Kidney(03) liver (04) lungs(05) Heart (06) Brain (07) Spleen (08) Mesenteric lymph nodes (09) Inguinal lymph nodes (10) Drug delivery site(11) Skin(12) Bone marrow (13) Bone marrow (thoracic )(14) Sciatic nerve (drug delivery site) (15) Salivary glands (16) Lacrimal gland (17) |
1248.48 824.18 357.34 199.15 323.04 144.98 81.67 518.42 670.75 1600.92 282.61 327.76 34.68 72.02 256.43 235.87 230.25 |
1.91 1.89 1.92 1.85 1.91 1.92 1.92 1.93 1.85 1.93 1.92 1.90 1.93 1.67 1.91 1.90 1.93 |
2.27 2.35 1.66 1.07 2.07 2.11 1.51 2.21 1.27 2.36 1.96 1.41 1.32 0.57 1.85 1.61 2.22 |
A total of nearly 8,000 samples were extracted using EE101, including samples such as urine, saliva, and skin swabs in addition to the above-mentioned sample types.
Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells[J]. Biosensors and Bioelectronics, 2015, 69:113-120.
Xu X , Wang S , Liu J , et al. Hypoxia induces downregulation of soluble guanylyl cyclase β1 by miR-34c-5p[J]. Journal of Cell Science, 2012, 125(24):6117-6126.
Geng J , Wang X , Wei H , et al. Efficient Attenuation of NK Cell-Mediated Liver Injury through Genetically Manipulating Multiple Immunogenes by Using a Liver-Directed Vector[J]. The Journal of Immunology, 2013, 190(9):4821-4829.
Yao P , Chen X , Yan Y , et al. Glutaredoxin 1, glutaredoxin 2, thioredoxin 1, and thioredoxin peroxidase 3 play important roles in antioxidant defense in Apis cerana cerana[J]. Free Radical Biology and Medicine, 2014, 68:335-346.
Luo J , Yang J , He H , et al. A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs[J]. Bioresource Technology, 2013, 139:141-148.