Benzonase Nuclease, No-specific endonuclease, 250 U/µl, is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease. It is a highly useful endonuclease for removal of nucleic acids DNA and RNA, from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
• It possesses a higher specific activity than other nucleases
• It is free of detectable proteolytic activities
• It can be used effectively in various fields of applications, including applications in biotechnological processing, such as recombinant protein expression and purification, and biopharmaceutical production • It has a proven track record in various commercial vaccine manufacturing processes. Bulk quantities are available. GMP grade is also available. See our GMP section for more information.