Size:100ug
Purification:Immunogen affinity purified
Form:liquid
Purity:?95% as determined by SDS-PAGE
Host:Rabbit
Clonality:polyclonal
Clone ID:
Isotype:IgG
Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background:DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.
Immunogen:zinc finger, RAN-binding domain containing 3
Synonyms:DNA annealing helicase and endonuclease ZRANB3,Annealing helicase 2,AH2
Observed MW:150 kDa and 50 kDa
Uniprot ID:Q5FWF4
Reactivity:Human
Tested Application:ELISA, WB
Recommended dilution:WB: 1:200-1:2000; IF: 1:20-1:200
Gene ID:84083
Research Area:Metabolism