GeneBio Systems

VIP ELISA kit (Human)

Name: VIP ELISA kit (Human)
Brand: GeneBio Systems
SKU: CEA380Hu
Price: 1093.00 USD
Availability: InStock

SKU:CEA380Hu

$1,093.00 USD

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Quantity

Size: 96Tests

# of Times Cited in literature: 19

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: VIP

Target Full Name: Vasoactive Intestinal Peptide

Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

Target Species: Human

Uniprot: P01282

Gene ID: 7432

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human VIP / Vasoactive Intestinal Peptide

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 6.17-500pg/mL

Sensitivity: 2.63pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP). No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vasoactive Intestinal Peptide (VIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vasoactive Intestinal Peptide (VIP) and unlabeled Vasoactive Intestinal Peptide (VIP) (Standards or samples) with the pre-coated antibody specific to Vasoactive Intestinal Peptide (VIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample.

Research Area: Endocrinology;Gastroenterology;Hormone metabolism;

References Citing This Product: VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population

Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy

CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine

Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine

Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients

Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters

Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis

Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients

No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine

Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.

Salivary neuropeptides, stress, and periodontitis