GeneBio Systems

TSH ELISA kit (Mouse)

Name: TSH ELISA kit (Mouse)
Brand: GeneBio Systems
SKU: CEA463Mu
Price: 1125.00 USD
Availability: InStock

SKU:CEA463Mu

$1,125.00 USD

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Quantity

Size: 96Tests

# of Times Cited in literature: 32

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: TSH

Target Full Name: Thyroid Stimulating Hormone

Alternative Names: Thyrotropin

Target Species: Mouse

Uniprot: P01216 & P12656

Gene ID: 12640

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Mouse TSH / Thyroid Stimulating Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 6.4-4,000pg/mL

Sensitivity: 2.5pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH). No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyroid Stimulating Hormone (TSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample.

Research Area: Endocrinology;Hormone metabolism;

References Citing This Product: Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID

Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function

Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver

Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis.

Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring

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Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population

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Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice