TCPa ELISA kit (General species)

SKU: CEA922Ge

Size: 96Tests

# of Times Cited in literature: 6

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: TCPa

Target Full Name: Alpha-Tocopherol

Alternative Names: VE; Vitamin E; α-TCP; α-Tocopherol

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species TCPa / Alpha-Tocopherol

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 1.23-100ug/mL

Sensitivity: 0.46ug/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Tocopherol (TCPa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Tocopherol (TCPa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Tocopherol (TCPa). No significant cross-reactivity or interference between Alpha-Tocopherol (TCPa) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Tocopherol (TCPa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Tocopherol (TCPa) and unlabeled Alpha-Tocopherol (TCPa) (Standards or samples) with the pre-coated antibody specific to Alpha-Tocopherol (TCPa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Tocopherol (TCPa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Tocopherol (TCPa) in the sample.

Research Area: Metabolic pathway;Nutrition metabolism;

References Citing This Product: Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile Men

Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats.

The potential effects of antioxidant feed additives in mitigating the adverse effects of cornnaturally contaminated with Fusarium mycotoxins on antioxidant systems in the intestinal mucosa, plasma, and liver in weaned pigs.

Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes

Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses

Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism

Prooxidant effect of α-tocopherol in pancreatic tumors.

Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study