Simian TAT(Thrombin/Antithrombin Complex) ELISA Kit

SKU: ELK11612

Size:96T

Reactivity:Simian

Alternative Names:Thrombin/Antithrombin Complex

Assay Type:Sandwich

Sensitivity:0.121 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Plasma

Assay length:3.5h

Research Area:Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TAT in the samples is then determined by comparing the OD of the samples to the standard curve.

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