Simian PSA(ProstateSpecific Antigen) ELISA Kit

SKU: ELK11532

Size:96T

Reactivity:Simian

Alternative Names:KLK3; APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein

Assay Type:Sandwich

Sensitivity:0.098 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian PSA in the samples is then determined by comparing the OD of the samples to the standard curve.

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