rT3(Reverse Triiodothyronine) ELISA Kit

SKU: ELK7898

Size:96T

Reactivity:General

Alternative Names:RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

Assay Type:Competitive Inhibition

Sensitivity:27.68 pg/mL

Standard:6000 pg/mL

Detection range:93.75-6000 pg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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