Recombinant Vibrio cholerae Toxin B Protein

SKU: PKSQ050090

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Choleragenoid;CTXB;toxB

Target Synonym: Cholera enterotoxin B chain;Cholera enterotoxin gamma chain;Cholera enterotoxin subunit B;Choleragenoid;ctxB;toxB

Research Areas:

Conjugation:

Target Species: Vibrio cholerae

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P01556

Accession: P01556

Background: Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Concentration:

Activity: Not validated for activity

Sequence: Thr22-Asn124

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 11.8 kDa

ObservedMW: 11 kDa