Recombinant Salmonella typhimurium Probable csgAB operon transcriptional regulatory protein (csgD)

SKU: O54294

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: O54294

Gene Names: csgD

Alternative Name(s):

Abbreviation: Recombinant Salmonella typhimurium csgD protein

Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Source: E.coli

Expression Region: 1-216aa

Protein Length: Full Length

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: MFNEVHSSHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQRSLEDISAGCIVLMDMMEADKKLIHYWQDNLSRKNNNIKTLLLNTPDDYPYREIENWPHINGVFYATEDQEHVVSGLQGILRGECYFSQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR

MW: 28.9 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Necessary for transcription of the csgAB operon. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription.

Reference: "Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain." Wen Y., Ouyang Z., Devreese B., He W., Shao Y., Lu W., Zheng F. Protein Sci 26: 2073-2082(2017)

Function: