Recombinant ROCK2+ROCK1 Monoclonal Antibody

SKU: AN301910L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:ROCK2+ROCK1

Target Synonym:Rho-associated protein kinase 2;ROCK-II;p160ROCK;ROCK-I

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A626

UNIProt ID:O75116;Q13464

Accession:

Background:ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity. ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain. ROCK2 is directly cleaved by granzyme B (grB). Cleavage activates ROCK2 and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction.

Concentration:1 mg/mL

Immunogen:Recombinant human ROCK2+ROCK1 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000;IHC 1:50-1:100;IF 1:50

Calculated MW:161, 158 kDa

ObservedMW:161 kDa