Recombinant rat Prostatic Acid Phosphatase/ACPP protein (His tag)

SKU: PDER100230

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:ACPP

Target Synonym:Acid phosphatase 3;Acp3;Fluoride-resistant acid phosphatase;Prostatic acid phosphatase;Protein tyrosine phosphatase ACP3;Thiamine monophosphatase (TMPase)

Research Areas:Cancer;Cell Biology

Conjugation:

Target Species:rat

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P20646

Accession:P20646

Background:ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP.

Concentration:

Activity:Not validated for activity

Sequence:Lys 32-Leu 381

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:38.4 kDa

ObservedMW:43 kDa