Recombinant Rat Occludin/OCLN protein (His tag)

SKU: PDER100187

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Occludin;OCLN

Target Synonym:OCLN_RAT;Occludin

Research Areas:Signal Transduction;Cell biology

Conjugation:

Target Species:Rat

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q6P6T5

Accession:Q6P6T5

Background:Rat Occludin is a 523 amino acid (aa), predicted molecular weight 59 kDa integral membrane protein that localizes within tight junctions of epithelial and endothelial cells. May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells. Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG), also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.

Concentration:

Activity:Not validated for activity

Sequence:Lys266-Thr522

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:28.2 kDa

ObservedMW:40 kDa