Recombinant Rat MIP-1 Beta/CCL4 protein

SKU: CD00086

Size:5 ug

Background:Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80 % and 86 % a.a. sequence identity with human and Mouse CCL4. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1-1 ng/ml.

Synonyms:MIP-1β/CCL4,Mip1-b, Scya4, Rat

Endotoxin:Less than 1 EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.

Storage:-20ºC

Sequence:APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN

uniprot accession:P523

Molecular weight:Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.