Recombinant Rat Midkine protein

SKU: CD00089

Size:5 ug

Background:Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family and it is encoded by the MDK gene. Midkine includes five intrachain disulfide bonds which held two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.2 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Mature rat midkine shares 89% and 98% a.a. sequence identity with human and Mouse.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Midkine

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1-1 ng/ml.

Synonyms:MDK,ARAP, MK, NEGF2,Midkine, Rat

Endotoxin:Less than 0.1 EU/μg of rRtMidkine as determined by LAL method.

Storage:-20ºC

Sequence:VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD

uniprot accession:Q9R1S9

Molecular weight:Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 12 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH7.4.