Recombinant Rat IL-9 protein

SKU: CD00099

Size:2 ug

Background:Interleukin-9 (IL-9) is encoded by the IL9 gene and produced by T-cells and specifically by CD4+ helper cells. IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLVI) transformed T cell line. It functions through the IL-9 receptor, which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 can support the growth of IL-2 independent and IL-4 independent helper T-cells. Rat IL-9 has approximately 73 % amino acid sequence identity with Mouse IL-9. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-9

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse TS1 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:IL-9, Rat

Endotoxin:Less than 0.1 EU/μg of rRtIL-9 as determined by LAL method.

Storage:-20ºC

Sequence:MQRCSTSWGI QHTSYLIENL KDDPSSKCSC SANVTSCLCL PIPSDDCTTP CFQEGMSQVT NATQQSKFSP FFFRVKRIVE TLKSNKCQFF SCEKPCNQTT AGNTVSFLKS LLKTFQKTEV QVQRSRA

uniprot accession:D4A8I9

Molecular weight:Approximately 14.3 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.