Recombinant Rat IL-17A protein

SKU: CD00110

Size:5 ug

Background:Interleukin-17A (IL-17A) belongs to the IL-17 family that contains IL-17A through IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, rat IL-17A exhibits 89 % amino acid identity with Mouse IL-17A. High levels of rat IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-17A

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inducing IL-6 secretion of Mouse NIH/3T3 cells is less than 1. ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:IL-17A,CTLA-8, IL-17, IL17A, Il17, Rat

Endotoxin:Less than 0.1 EU/μg of rRtIL-17A as determined by LAL method.

Storage:-20ºC

Sequence:AVLIPQSSVC PNAEANNFLQ NVKVNLKVIN SLSSKASSRR PSDYLNRSTS PWTLSRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPEKCPFT FRVEKMLVGV GCTCVSSIVR HAS

uniprot accession:Q61453

Molecular weight:Approximately 3. kDa, a disulfide-linked homodimer of two 133 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, pH 6.5.