Recombinant Rat GDNF protein

SKU: CD00122

Size:2 ug

Background:Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Glial Cell-derived

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using rat C6 cells is less than .2 ng/ml, corresponding to a specific activity of > 5. × 16 IU/mg.

Synonyms:GDNF,gndf, Rat

Endotoxin:Less than 1 EU/μg of rRtGDNF as determined by LAL method.

Storage:-20ºC

Sequence:MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

uniprot accession:Q7731

Molecular weight:Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, 5 mM NaCl, pH 6.4.