Recombinant Rat FGF-10/KGF-2 protein

SKU: CD00127

Size:5 ug

Background:FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Rat FGF-10 shares approximately 95 % amino acid sequence identity with human FGF-10. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Fibroblast Growth Factor-10

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 12 ng/ml, corresponding to a specific activity of > 8.3 × 13 IU/mg.

Synonyms:FGF-1/KGF-2, FGF1, Fgf5a,Rat

Endotoxin:Less than 1 EU/μg of rRtKGF-2/FGF-10 as determined by LAL method.

Storage:-20ºC

Sequence:QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

uniprot accession:P7492

Molecular weight:Approximately 2. kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris, 5 mM NaCl, pH 7.4, 5 % trehalose.