Recombinant Pim-1 Monoclonal Antibody

SKU: AN302025L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Pim-1

Target Synonym:PIM;PIM1;Oncogene PIM 1;Oncogene PIM1;PIM 1;Pim 1 kinase;pim 1 kinase 44 kDa isoform;Pim 1 oncogene;pim 1 oncogene (proviral integration site 1);pim1 kinase 44 kDa isoform;Pim2;PIM3;Proto oncogene serine/threonine protein kinase Pim 1;Proto-oncogene serine/threonine-protein kinase Pim-1;Proviral integration site 1;Proviral integration site 2

Research Areas:Signal Transduction;Stem Cells;Epigenetics and Nuclear Signaling;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A745

UNIProt ID:P11309

Accession:

Background:Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines. Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis. Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100. Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas. Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity. Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis.The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab.

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000

Calculated MW:36 kDa

ObservedMW:33, 34 kDa