Recombinant Pig Proenkephalin-B (PDYN), partial

SKU: P01214

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P01214

Gene Names: PDYN

Alternative Name(s): (Beta-neoendorphin-dynorphin)(Preprodynorphin)(Big Dyn)(1-17)(Dyn-A17)(Dynorphin A)(1-13)(1-8)(Dynorphin B)(Dyn-B)(Dynorphin B(1-13)(Dynorphin B-29)

Abbreviation: Recombinant Pig PDYN protein, partial

Organism: Sus scrofa (Pig)

Source: E.coli

Expression Region: 175-184aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-KSI-tagged

Target Protein Sequence: YGGFLRKYPK

MW: 16.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. ; Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.; Leumorphin has a typical opiod activity and may have anti-apoptotic effect.

Reference: "A new opioid octapeptide related to dynorphin from porcine hypothalamus." Minamino N., Kangawa K., Fukuda A., Matsuo H., Iagarashi M. Biochem. Biophys. Res. Commun. 95: 1475-1481(1980)

Function: