Recombinant Pig Glutamate carboxypeptidase 2 (FOLH1), partial, Biotinylated

SKU: O77564

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: O77564

Gene Names: FOLH1

Alternative Name(s): (Folate hydrolase 1)(Folylpoly-gamma-glutamate carboxypeptidase)(FGCP)(Glutamate carboxypeptidase II)(GCPII)(Membrane glutamate carboxypeptidase)(mGCP)(N-acetylated-alpha-linked acidic dipeptidase I)(NAALADase I)(Prostate-specific membrane antigen homolog)(Pteroylpoly-gamma-glutamate carboxypeptidase)

Abbreviation: Recombinant Pig FOLH1 protein, partial, Biotinylated

Organism: Sus scrofa (Pig)

Source: E.coli

Expression Region: 44-751aa

Protein Length: Partial

Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged

Target Protein Sequence: KSPNEAANISPQHNVKKAFLDELKAENIKTFLYNFTRIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTRPNYISIIDEDGNEIFNTSLFEPPPPGYENVSDVVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKILIARYGKIFRGNKVKNAQLAGAKGIILYSDPADYFAPGVQSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRLQIAEAVGLPRIPVHPIGYSDAQKLLEKMGGSAPPDDSWKGSLHVPYNVGPGFTGNFSTQKVKMHIHSDNKVKRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGKLKKEGWRPRRTVLFASWDAEEYGLFGSTEWAEENSRILQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLFESWNEKSPSPEFSGLPRISKLGSGNDFEVFFQRLGIASGRARYTKDWVTNKFSSYPLYHSVYETYELVEKFYDPTFKYHLAVAQVRGGIVFELANSVVRPFDCRDYAVVLRNYADKLYNISMNHPQEMKAYSVSFDSLFSAVKNFTEIASNFSERVQDLDKNNPILLRIMNDQLMFLERAFIVPLGLPDRAFYRHVIYAPSSHNKYMGESFPGIYDALFDIENKVDPSKAWGEVKRQISIAAFTVQAAAGTLREVA

MW: 128.0 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. ; Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Reference: "Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II." Halsted C.H., Ling E.-H., Luthi-Carter R., Villanueva J.A., Gardner J.M., Coyle J.T. J. Biol. Chem. 273: 20417-20424(1998)

Function: