Recombinant Ovine Interferon-tau protein

SKU: CD00156

Size:2 ug

Background:IFN-τ is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN-τ has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-α/β. The current investigation concerns the effect of recombinant ovine IFN-tau (rOvIFN-τ) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and up regulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS is via interference with IFN-γ-induced MHC class II expression. IFN-τwas shown to down regulate IFN-γ-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in down regulating inflammation in the central nervous system (CNS). IFN-τdid not upregulate the expression of MHC class II on CVE. IFN-τalso inhibited the replication of Theilers virus in CVE.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Pichia Pastoris

Full product name:Recombinant Ovine Interferon-tau

Biological activity:Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral resistance assay is no less than 1. × 17 IU/mg.

Synonyms:IFN-τ, IFN-tau-c2, IFNT, IFNT1, TP-1,IFNT2,Ovine

Endotoxin:Less than 0.1 EU/μg of rOvIFN-τ as determined by LAL method.

Storage:-20ºC

Sequence:CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ EMVEGDQLQK DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT LLEQLCTGLQ QQLDHLDTCR GQVMGEEDSE LGNMDPIVTV KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR ALTVSTTLQK RLTKMGGDLN SP

uniprot accession:P15696

Molecular weight:Approximately 19.9 kDa, a single glycosylated polypeptide chain containing 172 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.