Recombinant Ophiophagus hannah Cathelicidin-related peptide Oh-Cath

SKU: B6S2X2

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: B6S2X2

Gene Names: N/A

Alternative Name(s): Cathelicidin-related antimicrobial peptide;Oh_CRAMP;Vipericidin

Abbreviation: Recombinant Ophiophagus hannah Cathelicidin-related peptide Oh-Cath protein

Organism: Ophiophagus hannah (King cobra) (Naja hannah)

Source: E.coli

Expression Region: 162-191aa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 6xHis-SUMO-tagged

Target Protein Sequence: KFFKKLKNSVKKRAKKFFKKPRVIGVSIPF

MW: 21.8 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Potent antimicrobial peptide against Gram-negative (MIC=0.25 ug/ml against E.coli ATCC 25922, MIC=0.5 ug/ml against P.aeruginosa) and Gram-positive bacteria (MIC=64 ug/ml against E.faecalis, MIC=64 ug/ml against S.aureus). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane. Low hemolytic activities have been observed on mammalian cells.

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Function: