Recombinant Mouse VEGF120 protein

SKU: CD00161

Size:2 ug

Background:Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGFs normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the Mouse homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98 % a.a. sequence identity with corresponding regions of rat, 89 % with canine, feline, equine and porcine, and 87 % with human, ovine and bovine VEGF, respectively.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Vascular Endothelial Growth Factor120

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:VEGF12, Mouse

Endotoxin:Less than 1 EU/μg of rMuVEGF120 as determined by LAL method.

Storage:-20ºC

Sequence:MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

uniprot accession:Q731

Molecular weight:Approximately 28.4 kDa, a disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains (Met start).

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.