Recombinant Mouse TNF- Alpha protein

SKU: CD00195

Size:5 ug

Background:Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and Mouse TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Tumor Necrosis Factor-alpha

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cytotoxicity assay using Mouse L929 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg in the presence of actinomycin D.

Synonyms:TNF-α,DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf, Mouse

Endotoxin:Less than 1 EU/μg of rMuTNF-α as determined by LAL method.

Storage:-20ºC

Sequence:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

uniprot accession:P684

Molecular weight:Approximately 17.4 kDa. The recombinant Mouse TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.2.