Recombinant Mouse OSM protein

SKU: CD00279

Size:2 ug

Background:Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant Mouse OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Oncostatin-M

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse NIH-3T3 cells is less than 1. ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:OSM, Mouse

Endotoxin:Less than 1 EU/μg of rMuOSM as determined by LAL method.

Storage:-20ºC

Sequence:NRGCSNSSSQ LLSQLQNQAN LTGNTESLLE PYIRLQNLNT PDLRAACTQH SVAFPSEDTL RQLSKPHFLS TVYTTLDRVL YQLDALRQKF LKTPAFPKLD SARHNILGIR NNVFCMARLL NHSLEIPEPT QTDSGASRST TTPDVFNTKI GSCGFLWGYH RFMGSVGRVF REWDDGSTRS R

uniprot accession:P53347

Molecular weight:Approximately 2.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.