Recombinant Mouse M-CSF protein(His Tag)

SKU: PKSM041498

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: M-CSF

Target Synonym: Ath;Ath-;Ath-1;Ath1;CD134;CD134L;OX-40L;OX4;TNFSF4;TXGP1;Tnlg2b;Txgp;Txgp1l;gp3;gp34

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P07141

Accession: P07141

Background: Macrophage colony-stimulating factor 1（M-csf）is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

Concentration:

Activity: Measure by its ability to induce proliferation in NFS-60 cells.The ED₅₀ for this effect is <2 ng/mL.The specific activity of recombinant mouse M-CSF is approximately >5 x 10⁵ IU/mg.

Sequence: Lys32-Pro187

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.0 kDa

ObservedMW: 17-25 kDa