Recombinant Mouse IL1RL1/ST2 Protein (Fc Tag)

SKU: PKSM041051

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL1RL1;ST2

Target Synonym: STE;DER;Fit;Protein T;Protein ST;Lymphocyte antigen;Interleukin-1 receptor-like;suppression of tumorigenicity;IL1RL;IL-1 R;IL-1RL;DER4;Fit-1;IL-1 R4;IL-1RL1;IL-33R;Interleukin-1 receptor-like 1;Lymphocyte antigen 84;Protein ST2;Protein T1;ST2L;ST2V;suppression of tumorigenicity 2;Ly84;St2;Ste2;Il1rl1

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc

UNIProt ID: P14719

Accession: P14719-2

Background: ST2, also called IL-1 R4, is an Interleukin-1 receptor family glycoprotein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This receptor is very similar to the IL-1 receptor type I and the IL-18 receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2). ST2L acts as a transmembrane signalling receptor for IL-33 by mediating the effect of IL-33 on the inflammatory process, while sST2 can suppress IL-33 activity.

Concentration:

Activity: Immobilized Mouse IL-33(Cat: PKSM041090) at 5μg/ml(100 μl/well) can bind Mouse ST2-Fc. The ED₅₀ of Mouse ST2-Fc is 0.25ug/ml.

Sequence: Ser27-Ala337(Val192Ala)

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.7 kDa

ObservedMW: 90-110 kDa