Recombinant Mouse IFN- Lambda2/IL-28A protein

SKU: CD00408

Size:5 ug

Background:Mouse IFN-lambda 2 (IL-28A), IFN-lambda 3 (IL-28B) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid sequence identity) and type I IFN family (15 - 19% a.a. sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28A shares 64%, 65%, and 56% a.a. identity with human IL-28A, IL-28B, and IL-29, respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interferon-lambda 2/Interleukin-28A

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 15 IU/mg.

Synonyms:IFN-λ2/IL-28A, Il; Il28a; IL-28A; If1ia1; EG33496,Ifnl2,Mouse

Endotoxin:Less than 0.1 EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method.

Storage:-20ºC

Sequence:DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV

uniprot accession:Q4VK74;B9EK98

Molecular weight:Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.