Recombinant Mouse Fractalkine/CX3CL1 protein

SKU: CD00442

Size:5 ug

Background:CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Mouse CX3CL1 contains 76 amino acids and it shares approximately 85 % and 77 % amino acid sequence homology with the rat and human protein.

Research_area:Others

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Fractalkine/CX3CL1

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than .5 μg/ml, corresponding to a specific activity of > 2 IU/mg.

Synonyms:Fractalkine/CX3CL1, FK; CX3C; Cxc3; Scyd; Scyd1; fract; neuro; ABCD-3; AB3188; AI848747; D8Bwg439e,Mouse

Endotoxin:Less than 0.1 EU/μg of rMuFractalkine/CX3CL1 as determined by LAL method.

Storage:-20ºC

Sequence:QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG

uniprot accession:O35188;D6RCV;ECXC3

Molecular weight:Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Mouse Fractalkine.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.